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KMID : 1094720080130030319
Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 3 p.319 ~ p.324
Efficient release of ferulic acid from sweet potato ( Ipomoea batatas ) stems by chemical hydrolysis
Choi Myung-Suk

Park Ji-Hyun
Min Ji-Yun
Lim Bu-Kug
Lee Byung-Hyun
Jung Gang-Won
Lee Jong-Yoon
Karigar Chandrakant S.
Yang Jae-Kyung
Abstract
An efficient method for release of ferulic acid from sweet potato stems was developed. Ferulic acid along with phenolic compounds were released from stems by acid and alkaline treatments. The base hydrolysis with 0.1 N NaOH yielded the highest quantity of total extracts (471.1 mg/g). The stems released more phenolic compounds when 0.0125¡­0.025 N NaOH was employed. Where as ferulic acid release was maximal with 0.05 N H2SO4 (0.32 mg/g). Ferulic acid was separated from phenolics by column chromatography. Among the elution solvents, ethyl acetate fractions (80%) contained ferulic acid. Ethyl acetate eluants were further fractionated with n-hexane/ethyl acetate/formic acid (100/50/0.5, v/v/v). All fractions showed ferulic acid and phenolic compounds. Fraction V among them was ascribed to ferulic acid with an yield of 5.41 mg/g of dry sweet potato tissue.
KEYWORD
ferulic acid, phenolic compounds, sweet potato, acid/base hydrolysis
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